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Hands-on Practical RNA-Seq Data Analysis With Galaxy

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If you’ve ever been curious about understanding the inner workings of RNA-Seq and Next-Generation Sequencing (NGS) technologies, this comprehensive course is tailored specifically for you. Over the course of several engaging sessions, we will take you on a journey through the intricate process of analyzing genetic data.

Course Contents:
Introduction to NGS, RNA-Seq Pipeline & GALAXY
  • Introduction to Next Generation Sequencing & RNA-Seq
  • RNA-Seq Data Analysis Workflow
  • What is Galaxy
  • How to Get Started With Galaxy Account 
Practical RNA-Seq Differential Gene Expression Analysis
  • RNA-Seq Dataset Retrieval
  • Quality Control with FastQC
  • Pro-processing of the Reads
  • Alignment of the Reads Against Reference Genome
  • Post Alignment Processing
  • Transcript Assembly and Quantification with StringTie
  • Differential Gene Expression Analysis with DESEQ2

At the outset of this course, you will lay the foundation for your understanding the fundamental principles of RNA-Seq and NGS. You will grasp the core concepts that underlie this cutting-edge technology, ensuring that you have a solid grasp of the theory behind it.

As we progress, you will transition from theory to practice, gaining practical skills that are essential for working with genetic data. You will learn how to perform crucial Quality Control and Trimming tasks using popular tools like FASTQC, FastP, and Cutadapt. These steps are vital to ensure the reliability of your data before diving into deeper analysis.

Next, you’ll learn about mapping and alignment and using HISAT2 to align sequencing data to a reference genome, and you’ll also learn how to evaluate the efficiency of this alignment. This step is critical for accurately identifying the location of genetic sequences within the genome.

Finally, you’ll explore the world of functional analysis. You’ll use Gene Ontology and KEGG Pathways to interpret the biological significance of your findings, allowing you to extract valuable insights from your RNA-Seq data. This step is crucial for understanding the biological processes and pathways affected by the gene expression changes you’ve identified.

Don’t miss out on this unparalleled opportunity to enhance your bioinformatics skills and make a significant impact in the world of genomics. By the end of this course, you’ll be equipped with the knowledge and practical skills needed to confidently tackle complex RNA-Seq and NGS data analysis projects, making you a valuable asset in the ever-evolving field of genomics research. Join us on this exciting journey of discovery and empowerment!


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What Will You Learn?

  • Introduction to RNA-Seq, NGS & Galaxy
  • Quality Control and Trimming Using FASTQC, FastP, Cutadapt
  • Mapping With HISAT2 & Evaluation of Alignment
  • Discovering DEGs with StringTie, DESeq2
  • Functional Analysis with Gene Ontology & KEGG Pathways

Course Content

Introduction to NGS, RNA-Seq Pipeline & GALAXY

Practical RNA-Seq Differential Gene Expression Analysis

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8 months ago
Clear and efficient.

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